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Goat anti-HAP1

EB07528
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHAP1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HAP1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9001
  • Target name
    HAP1
  • Target description
    huntingtin associated protein 1
  • Target synonyms
    HAP2, HIP5, HLP, hHLP1, huntingtin-associated protein 1, HAP-1, epididymis secretory sperm binding protein, huntingtin-associated protein 2, neuroan 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003940.2; NP_817084.1. Purification: Antigen affinity purified. Names and symbols: HAP1; huntingtin-associated protein 1 (neuroan 1); HAP2; HIP5; HLP; hHLP1; huntingtin-associated protein 1; huntingtin-associated protein 2; neuroan 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HAP1 using anti-HAP1 antibody (A01658-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAP1 antigen affinity purified polyclonal antibody (Catalog # A01658-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAP1 at approximately 80KD. The expected band size for HAP1 is at 80KD.
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