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Goat anti-HNF1A

EB06701
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHNF1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HNF1A Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the Nucleus of NIH3T3 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6927
  • Target name
    HNF1A
  • Target description
    HNF1 homeobox A
  • Target synonyms
    HNF-1-alpha, HNF-1A, HNF1, HNF1alpha, HNF4A, IDDM20, LFB1, MODY3, TCF-1, TCF1, hepatocyte nuclear factor 1-alpha, albumin proximal factor, hepatic nuclear factor 1, interferon production regulator factor, liver-specific transcription factor LF-B1, transcription factor 1, hepatic
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000536.5. GeneIDs all Nonhuman: 21405 (mouse); 24817 (rat);. Purification: Antigen affinity purified. Names and symbols: HNF1A; HNF1 homeobox A; HNF-1A; HNF1; TCF1; TCF-1; IDDM20; LFB1; MODY3; HNF-1-alpha; OTTHUMP00000198944; OTTHUMP00000240200; OTTHUMP00000240204; OTTHUMP00000240206; albumin proximal factor; hepatic nuclear factor 1; hepatocyte nuclear factor 1-alpha; inte
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HNF1/HNF1A using anti-HNF1/HNF1A antibody (PB9214). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1/HNF1A antigen affinity purified polyclonal antibody (Catalog # PB9214) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1/HNF1A at approximately 80 kDa. The expected band size for HNF1/HNF1A is at 67 kDa.
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