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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse liver tissue, Primary antibody: CSB-PA944576(HNF1A Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 45 seconds
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse liver tissue, Primary antibody: CSB-PA944576(HNF1A Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 45 seconds
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse liver tissue, Primary antibody: CSB-PA944576(HNF1A Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 45 seconds

HNF1A Antibody

CSB-PA944576
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNF1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNF1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6927
  • Target name
    HNF1A
  • Target description
    HNF1 homeobox A
  • Target synonyms
    HNF-1-alpha, HNF-1A, HNF1, HNF1alpha, HNF4A, IDDM20, LFB1, MODY3, TCF-1, TCF1, hepatocyte nuclear factor 1-alpha, albumin proximal factor, hepatic nuclear factor 1, interferon production regulator factor, liver-specific transcription factor LF-B1, transcription factor 1, hepatic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20823
  • Protein Name
    Hepatocyte nuclear factor 1-alpha
  • Scientific Description
    The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5-GTTAATNATTAAC-3. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HNF1/HNF1A using anti-HNF1/HNF1A antibody (PB9214). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1/HNF1A antigen affinity purified polyclonal antibody (Catalog # PB9214) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1/HNF1A at approximately 80 kDa. The expected band size for HNF1/HNF1A is at 67 kDa.
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