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ProductsBack/Goat anti-kallikrein 6

Goat anti-kallikrein 6

EB12265
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKLK6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-kallikrein 6 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5653
  • Target name
    KLK6
  • Target description
    kallikrein related peptidase 6
  • Target synonyms
    Bssp, Klk7, PRSS18, PRSS9, SP59, hK6, kallikrein-6, neurosin, protease M, serine protease 18, serine protease 9, zyme
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002765.1; NP_001012983.1. Purification: Antigen affinity purified. Names and symbols: KLK6; kallikrein-related peptidase 6; Bssp; Klk7; PRSS18; PRSS9; SP59; hK6; kallikrein-6; neurosin; protease M; serine protease 18; serine protease 9; zyme
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kallikrein 6/KLK6 using anti-Kallikrein 6/KLK6 antibody (A01882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 6/KLK6 antigen affinity purified polyclonal antibody (Catalog # A01882-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 6/KLK6 at approximately 29 kDa. The expected band size for Kallikrein 6/KLK6 is at 27 kDa.
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Western blot All lanes: KLK6 antibody at 5microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 16, 5 kDa Observed band size: 27 kDa
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