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Western blot All lanes: KLK6 antibody at 5microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 16, 5 kDa Observed band size: 27 kDa
Western blot All lanes: KLK6 antibody at 5microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 16, 5 kDa Observed band size: 27 kDa
Western blot All lanes: KLK6 antibody at 5microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 16, 5 kDa Observed band size: 27 kDa

KLK6 Antibody

CSB-PA842165LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKLK6
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Overview

  • Supplier
    Cusabio
  • Product Name
    KLK6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5653
  • Target name
    KLK6
  • Target description
    kallikrein related peptidase 6
  • Target synonyms
    Bssp, Klk7, PRSS18, PRSS9, SP59, hK6, kallikrein-6, neurosin, protease M, serine protease 18, serine protease 9, zyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92876
  • Protein Name
    Kallikrein-6
  • Scientific Description
    Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kallikrein 6/KLK6 using anti-Kallikrein 6/KLK6 antibody (A01882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 6/KLK6 antigen affinity purified polyclonal antibody (Catalog # A01882-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 6/KLK6 at approximately 29 kDa. The expected band size for Kallikrein 6/KLK6 is at 27 kDa.
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