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ProductsBack/KLK6 / Kallikrein 6 Antibody (Preservative Free)

KLK6 / Kallikrein 6 Antibody (Preservative Free)

LS-C743824
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetKLK6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KLK6 / Kallikrein 6 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ELISA
  • Applications Supplier
    ELISA (1:640000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5653
  • Target name
    KLK6
  • Target description
    kallikrein related peptidase 6
  • Target synonyms
    Bssp; hK6; kallikrein-6; Klk7; neurosin; protease M; PRSS18; PRSS9; serine protease 18; serine protease 9; SP59; zyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with KLK6 Polyclonal Antibody (bs-5870R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Figure 1. Western blot analysis of Kallikrein 6/KLK6 using anti-Kallikrein 6/KLK6 antibody (A01882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 6/KLK6 antigen affinity purified polyclonal antibody (Catalog # A01882-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 6/KLK6 at approximately 29 kDa. The expected band size for Kallikrein 6/KLK6 is at 27 kDa.
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