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Goat anti-KCNQ5

EB09212
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetKCNQ5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-KCNQ5 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID56479
  • Target name
    KCNQ5
  • Target description
    potassium voltage-gated channel subfamily Q member 5
  • Target synonyms
    KQT-like 5; Kv7.5; MRD46; potassium channel protein; potassium channel subunit alpha KvLQT5; potassium channel, voltage gated KQT-like subfamily Q, member 5; potassium voltage-gated channel subfamily KQT member 5; voltage-gated potassium channel subunit Kv7.5
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_062816.2; NP_001153602.1; NP_001153604.1; NP_1153605.1; NP_001153606.1. GeneIDs all Nonhuman: 226922 (mouse); 259273 (rat);. Purification: Antigen affinity purified. Names and symbols: KCNQ5; potassium voltage-gated channel, KQT-like subfamily, member 5; Kv7.5; OTTHUMP00000016729; OTTHUMP00000064152; OTTHUMP00000064153; potassium channel protein
  • Reactivity Supplier
    Human, Mouse, Rat, Pig, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ5 antigen affinity purified polyclonal antibody (Catalog # A04705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNQ5 at approximately 102KD. The expected band size for KCNQ5 is at 102KD.
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