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KCNQ5 antibody [N3C2], Internal

GTX106198
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetKCNQ5
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Overview

  • Supplier
    GeneTex
  • Product Name
    KCNQ5 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.45 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56479
  • Target name
    KCNQ5
  • Target description
    potassium voltage-gated channel subfamily Q member 5
  • Target synonyms
    KQT-like 5; Kv7.5; MRD46; potassium channel protein; potassium channel subunit alpha KvLQT5; potassium channel, voltage gated KQT-like subfamily Q, member 5; potassium voltage-gated channel subfamily KQT member 5; voltage-gated potassium channel subunit Kv7.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR82
  • Protein Name
    Potassium voltage-gated channel subfamily KQT member 5
  • Scientific Description
    This gene is a member of the KCNQ potassium channel gene family that is differentially expressed in subregions of the brain and in skeletal muscle. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of only one has been determined. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ5 antigen affinity purified polyclonal antibody (Catalog # A04705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNQ5 at approximately 102KD. The expected band size for KCNQ5 is at 102KD.
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