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KCNQ5 Antibody

CSB-PA040010
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetKCNQ5
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNQ5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56479
  • Target name
    KCNQ5
  • Target description
    potassium voltage-gated channel subfamily Q member 5
  • Target synonyms
    Kv7.5, MRD46, potassium voltage-gated channel subfamily KQT member 5, KQT-like 5, potassium channel protein, potassium channel subunit alpha KvLQT5, potassium channel, voltage gated KQT-like subfamily Q, member 5, voltage-gated potassium channel subunit Kv7.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR82
  • Protein Name
    Potassium voltage-gated channel subfamily KQT member 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ5 antigen affinity purified polyclonal antibody (Catalog # A04705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNQ5 at approximately 102KD. The expected band size for KCNQ5 is at 102KD.
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