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ProductsBack/Goat anti-LXR beta / NR1H2 Antibody

Goat anti-LXR beta / NR1H2 Antibody

EB06521
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetNR1H2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-LXR beta / NR1H2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7376
  • Target name
    NR1H2
  • Target description
    nuclear receptor subfamily 1 group H member 2
  • Target synonyms
    LXR-b, LXRB, NER, NER-I, RIP15, UNR, oxysterols receptor LXR-beta, LX receptor beta, liver X nuclear receptor beta, nuclear orphan receptor LXR-beta, nuclear receptor NER, steroid hormone-nuclear receptor NER, ubiquitously-expressed nuclear receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_009052.3. Purification: Antigen affinity purified. Names and symbols: NR1H2; LXR-b; NER; UNR; NER-I; RIP15; nuclear receptor subfamily 1, group H, member 2; ubiquitously-expressed nuclear receptor; LXRB; LX receptor beta; liver X receptor beta; liver X receptor-beta; nuclear orphan receptor LXR-beta; oxysterols receptor LXR
  • Reactivity
    Canine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LXR beta/NER/NR1H2 using anti-LXR beta/NER/NR1H2 antibody (A04523-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LXR beta/NER/NR1H2 antigen affinity purified polyclonal antibody (Catalog # A04523-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LXR beta/NER/NR1H2 at approximately 50KD. The expected band size for LXR beta/NER/NR1H2 is at 50KD.
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