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ProductsBack/LXR beta antibody [N1], N-term

LXR beta antibody [N1], N-term

GTX104800
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNR1H2
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Overview

  • Supplier
    GeneTex
  • Product Name
    LXR beta antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.84 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7376
  • Target name
    NR1H2
  • Target description
    nuclear receptor subfamily 1 group H member 2
  • Target synonyms
    liver X nuclear receptor beta; LX receptor beta; LXRB; LXR-b; NER; NER-I; nuclear orphan receptor LXR-beta; nuclear receptor NER; oxysterols receptor LXR-beta; RIP15; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor; UNR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55055
  • Protein Name
    Oxysterols receptor LXR-beta
  • Scientific Description
    The LX receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor (see MIM 180245) and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides. Two genes, alpha (LXRA, MIM 602423) and beta, are known to encode LXR proteins (Song et al., 1995 [PubMed 7625741]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LXR beta/NER/NR1H2 using anti-LXR beta/NER/NR1H2 antibody (A04523-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LXR beta/NER/NR1H2 antigen affinity purified polyclonal antibody (Catalog # A04523-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LXR beta/NER/NR1H2 at approximately 50KD. The expected band size for LXR beta/NER/NR1H2 is at 50KD.
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