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ProductsBack/LXR alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated

LXR alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated

BS-10311R-BF350
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNR1H2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LXR alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Other Conjugate
  • Gene ID7376
  • Target name
    NR1H2
  • Target description
    nuclear receptor subfamily 1 group H member 2
  • Target synonyms
    liver X nuclear receptor beta; LX receptor beta; LXRB; LXR-b; NER; NER-I; nuclear orphan receptor LXR-beta; nuclear receptor NER; oxysterols receptor LXR-beta; RIP15; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor; UNR
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LXR beta/NER/NR1H2 using anti-LXR beta/NER/NR1H2 antibody (A04523-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LXR beta/NER/NR1H2 antigen affinity purified polyclonal antibody (Catalog # A04523-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LXR beta/NER/NR1H2 at approximately 50KD. The expected band size for LXR beta/NER/NR1H2 is at 50KD.
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