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ProductsBack/Goat anti-Myogenin / MYF4

Goat anti-Myogenin / MYF4

EB09955
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMYOG
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Myogenin / MYF4 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4656
  • Target name
    MYOG
  • Target description
    myogenin
  • Target synonyms
    MYF4, bHLHc3, myf-4, myogenin, class C basic helix-loop-helix protein 3, myogenic factor 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002470.2. Purification: Antigen affinity purified. Names and symbols: MYOG; myogenin (myogenic factor 4); MYF4; MYOGENIN; bHLHc3; Myogenic factor-4; myogenin; myogenic factor 4; myogenin
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myogenin/MYOG using anti-Myogenin/MYOG antibody (A03238-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myogenin/MYOG antigen affinity purified polyclonal antibody (Catalog # A03238-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myogenin/MYOG at approximately 40 kDa. The expected band size for Myogenin/MYOG is at 25 kDa.
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