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Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti MYOG/MYOGENIN Polyclonal Antibody,Unconjugated (bs-3550R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti MYOG/MYOGENIN Polyclonal Antibody,Unconjugated (bs-3550R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti MYOG/MYOGENIN Polyclonal Antibody,Unconjugated (bs-3550R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Myogenin Polyclonal Antibody

bs-3550R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMYOG
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Overview

  • Supplier
    Bioss
  • Product Name
    Myogenin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500), ICC/IF(ICC/IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4656
  • Target name
    MYOG
  • Target description
    myogenin
  • Target synonyms
    MYF4, bHLHc3, myf-4, myogenin, class C basic helix-loop-helix protein 3, myogenic factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15173
  • Protein Name
    Myogenin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang Y, Liu S, Yan Y, et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019,10(11):852. doi: 10.1038/s41419-019-2049-4
    Read this paper
  • Li S, Fu Y, Pang Y, et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019,234(11):21211-21223. doi: 10.1002/jcp.28727
    Read this paper

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Figure 1. Western blot analysis of Myogenin/MYOG using anti-Myogenin/MYOG antibody (A03238-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myogenin/MYOG antigen affinity purified polyclonal antibody (Catalog # A03238-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myogenin/MYOG at approximately 40 kDa. The expected band size for Myogenin/MYOG is at 25 kDa.
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