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Goat anti-NANOG

EB06860
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNanog
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NANOG Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483674 and 18931654. This antibody was successfully used in ICC on Human: PMID: 31479876, 29034898 and 29034891. Enzyme immunoassay: This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID71950
  • Target name
    Nanog
  • Target description
    Nanog homeobox
  • Target synonyms
    2410002E02Rik, ENK, Stm1, ecat4, homeobox protein NANOG, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox transcription factor Nanog
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_079141.2. GeneIDs all Nonhuman: 71950 (mouse); 414065 (rat);. Purification: Antigen affinity purified. Names and symbols: NANOG; Nanog homeobox; HGNC:20857 ; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
  • Reactivity Supplier
    Human, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Malerba N, Benzoni P, Squeo GM, et al. Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line. Stem Cell Res. 2019,40:101547. doi: 10.1016/j.scr.2019.101547
    Read this paper
  • Arellano-Viera E, Zabaleta L, Castaño J, et al. Generation of two transgene-free human iPSC lines from CD133(+) cord blood cells. Stem Cell Res. 2019,36:101410. doi: 10.1016/j.scr.2019.101410
    Read this paper
  • Mora C, Serzanti M, Giacomelli A, et al. Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a PITX2 p.M200V mutation. Stem Cell Res. 2017,24:8-11. doi: 10.1016/j.scr.2017.08.007
    Read this paper
  • Mora C, Serzanti M, Giacomelli A, et al. Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a KCNA5 p.D322H mutation. Stem Cell Res. 2017,24:29-32. doi: 10.1016/j.scr.2017.08.009
    Read this paper
  • Sancho-Martinez I, Nivet E, Xia Y, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016,7:10743. doi: 10.1038/ncomms10743
    Read this paper
  • Tolar J, McGrath JA, Xia L, et al. Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa. J Invest Dermatol. 2014,134(5):1246-1254. doi: 10.1038/jid.2013.523
    Read this paper
  • Montserrat N, Ramírez-Bajo MJ, Xia Y, et al. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2. J Biol Chem. 2012,287(29):24131-8. doi: 10.1074/jbc.M112.350413
    Read this paper
  • Vassena R, Montserrat N, Carrasco Canal B, et al. Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells. Hum Mol Genet. 2012,21(15):3366-73. doi: 10.1093/hmg/dds168
    Read this paper
  • Montserrat N, de Oñate L, Garreta E, et al. Generation of feeder-free pig induced pluripotent stem cells without Pou5f1. Cell Transplant. 2012,21(5):815-25. doi: 10.3727/096368911X601019
    Read this paper
  • Sánchez-Danés A, Consiglio A, Richaud Y, et al. Efficient generation of A9 midbrain dopaminergic neurons by lentiviral delivery of LMX1A in human embryonic stem cells and induced pluripotent stem cells. Hum Gene Ther. 2012,23(1):56-69. doi: 10.1089/hum.2011.054
    Read this paper

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 37 kDa. The expected band size for Nanog is at 37 kDa.
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