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Goat anti-NANOG

EB06860
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNanog
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NANOG Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483674 and 18931654. This antibody was successfully used in ICC on Human: PMID: 31479876, 29034898 and 29034891. Enzyme immunoassay: This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID71950
  • Target name
    Nanog
  • Target description
    Nanog homeobox
  • Target synonyms
    2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; ecat; ecat4; EN; ENK; ES cell-associated protein 4; homeobox protein NANOG; homeobox transcription factor Nanog
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_079141.2. GeneIDs all Nonhuman: 71950 (mouse); 414065 (rat);. Purification: Antigen affinity purified. Names and symbols: NANOG; Nanog homeobox; HGNC:20857 ; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
  • Reactivity Supplier
    Human, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line. Malerba N et al., 2019 Oct, Stem Cell Res
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  • Generation of two transgene-free human iPSC lines from CD133+ cord blood cells. Arellano-Viera E et al., 2019 Apr, Stem Cell Res
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  • Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a PITX2 p.M200V mutation. Mora C et al., 2017 Oct, Stem Cell Res
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  • Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a KCNA5 p.D322H mutation. Mora C et al., 2017 Oct, Stem Cell Res
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  • Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Sancho-Martinez I et al., 2016 Feb 22, Nat Commun
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  • Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa. Tolar J et al., 2014 May, J Invest Dermatol
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  • Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2. Montserrat N et al., 2012 Jul 13, J Biol Chem
    Read more
  • Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells. Vassena R et al., 2012 Aug 1, Hum Mol Genet
    Read more
  • Generation of feeder-free pig induced pluripotent stem cells without Pou5f1. Montserrat N et al., 2012, Cell Transplant
    Read more
  • Efficient generation of A9 midbrain dopaminergic neurons by lentiviral delivery of LMX1A in human embryonic stem cells and induced pluripotent stem cells. Sanchez-Danes A et al., 2012 Jan, Hum Gene Ther
    Read more

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 37 kDa. The expected band size for Nanog is at 37 kDa.
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