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Rabbit anti-Nanog Antibody, Affinity Purified

A300-397A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNanog
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Nanog Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID71950
  • Target name
    Nanog
  • Target description
    Nanog homeobox
  • Target synonyms
    2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; ecat; ecat4; EN; ENK; ES cell-associated protein 4; homeobox protein NANOG; homeobox transcription factor Nanog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ80Z64
  • Protein Name
    Homeobox protein NANOG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 37 kDa. The expected band size for Nanog is at 37 kDa.
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