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ProductsBack/Goat anti-Neuroserpin

Goat anti-Neuroserpin

EB06688
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSERPINI1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Neuroserpin Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5274
  • Target name
    SERPINI1
  • Target description
    serpin family I member 1
  • Target synonyms
    HNS-S1, HNS-S2, PI12, neuroserpin, neuroserpin, PI-12, peptidase inhibitor 12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1, serpin I1, serpin peptidase inhibitor clade I member 1, serpin peptidase inhibitor, clade I (neuroserpin), member 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005016.1. Purification: Antigen affinity purified. Names and symbols: SERPINI1; neuroserpin; PI12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1; protease inhibitor 12 (neuroserpin); serpin peptidase inhibitor, clade I (neuroserpin), member 1; DKFZp781N13156
  • Reactivity Supplier
    Human, Mouse, Rat, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Neuroserpin using anti-Neuroserpin antibody (PB9744). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuroserpin antigen affinity purified polyclonal antibody (Catalog # PB9744) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuroserpin at approximately 46 kDa. The expected band size for Neuroserpin is at 46 kDa.
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