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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA859934LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA859934LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA859934LA01HU at dilution of 1:100

SERPINI1 Antibody

CSB-PA859934LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSERPINI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5274
  • Target name
    SERPINI1
  • Target description
    serpin family I member 1
  • Target synonyms
    HNS-S1; HNS-S2; neuroserpin; peptidase inhibitor 12; PI12; PI-12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1; serpin I1; serpin peptidase inhibitor clade I member 1; serpin peptidase inhibitor, clade I (neuroserpin), member 1; truncated HNS-T
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99574
  • Protein Name
    Neuroserpin
  • Scientific Description
    Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neuroserpin using anti-Neuroserpin antibody (PB9744). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuroserpin antigen affinity purified polyclonal antibody (Catalog # PB9744) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuroserpin at approximately 46 kDa. The expected band size for Neuroserpin is at 46 kDa.
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