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ProductsBack/Goat anti-NFATC2 / NFAT1

Goat anti-NFATC2 / NFAT1

EB09503
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNFATC2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NFATC2 / NFAT1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4773
  • Target name
    NFATC2
  • Target description
    nuclear factor of activated T cells 2
  • Target synonyms
    NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NF-ATc2; NFATP; nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036472.2; NP_775114.1; NP_001129493.1. GeneIDs all Nonhuman: 140488 (mouse); 312320 (rat);. Purification: Antigen affinity purified. Names and symbols: NFATC2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; KIAA0611; NFAT1; NFATP; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; OTTHUMP00000031291; T cell transcription factor NFAT1; nuclear factor
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFAT1 using anti-NFAT1 antibody (A00969). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT1 antigen affinity purified polyclonal antibody (Catalog # A00969) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT1 at approximately 100KD. The expected band size for NFAT1 is at 100KD.
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