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ProductsBack/NFATc2 Recombinant Antibody, AbBy Fluor-350 Conjugated

NFATc2 Recombinant Antibody, AbBy Fluor-350 Conjugated

BSM-61447R-BF350
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetNFATC2
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Overview

  • Supplier
    Bioss
  • Product Name
    NFATc2 Recombinant Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID4773
  • Target name
    NFATC2
  • Target description
    nuclear factor of activated T cells 2
  • Target synonyms
    JCOSL, NFAT1, NFATP, nuclear factor of activated T-cells, cytoplasmic 2, NF-ATc2, NFAT pre-existing subunit, NFAT transcription complex, preexisting component, T cell transcription factor NFAT1, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, nuclear factor of activated T-cells, preexisting component, preexisting nuclear factor of activated T-cells 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13469
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFAT1 using anti-NFAT1 antibody (A00969). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT1 antigen affinity purified polyclonal antibody (Catalog # A00969) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT1 at approximately 100KD. The expected band size for NFAT1 is at 100KD.
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