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GRIN2B Antibody (YA4228, Mouse)

HY-P84531
MedChem Express
TargetGRIN2B
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GRIN2B Antibody (YA4228, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2904
  • Target name
    GRIN2B
  • Target description
    glutamate ionotropic receptor NMDA type subunit 2B
  • Target synonyms
    DEE27, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, NR3, hNR3, glutamate receptor ionotropic, NMDA 2B, GluN2B(alt_5'UTR), N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    GRIN2B Antibody (YA4228) is a mouse-derived and non-conjugated IgG1 antibody, targeting to GRIN2B. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NMDAR2B using anti-NMDAR2B antibody (PB9373). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR2B antigen affinity purified polyclonal antibody (Catalog # PB9373) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR2B at approximately 180-190 kDa. The expected band size for NMDAR2B is at 166 kDa.
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