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Goat anti-NTE

EB09135
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetPNPLA6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NTE Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10908
  • Target name
    PNPLA6
  • Target description
    patatin like phospholipase domain containing 6
  • Target synonyms
    BNHS; iPLA2delta; LNMS; neuropathy target esterase; NTE; NTEMND; OMCS; patatin-like phospholipase domain-containing protein 6; SPG39; sws
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006693.3. GeneIDs all Nonhuman: 50767 (mouse);. Purification: Antigen affinity purified. Names and symbols: PNPLA6; patatin-like phospholipase domain containing 6; NTE; NTEMND; SPG39; sws; neuropathy target esterase
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PNPLA6 using anti-PNPLA6 antibody (A04071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA6 antigen affinity purified polyclonal antibody (Catalog # A04071-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA6 at approximately 150 kDa. The expected band size for PNPLA6 is at 150,151 kDa.
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