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Western Blot analysis of AD293 cells using NTE Polyclonal Antibody.
Western Blot analysis of AD293 cells using NTE Polyclonal Antibody.
Western Blot analysis of AD293 cells using NTE Polyclonal Antibody.

PNPLA6 Antibody

CSB-PA005585
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPNPLA6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PNPLA6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10908
  • Target name
    PNPLA6
  • Target description
    patatin like domain 6, lysophospholipase
  • Target synonyms
    BNHS, LNMS, NTE, NTEMND, OMCS, SPG39, iPLA2delta, sws, patatin-like phospholipase domain-containing protein 6, neuropathy target esterase, patatin like phospholipase domain containing 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IY17
  • Protein Name
    Patatin-like phospholipase domain-containing protein 6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PNPLA6 using anti-PNPLA6 antibody (A04071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA6 antigen affinity purified polyclonal antibody (Catalog # A04071-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA6 at approximately 150 kDa. The expected band size for PNPLA6 is at 150,151 kDa.
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