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Goat anti-PGK1

EB12390
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPGK1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PGK1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5230
  • Target name
    PGK1
  • Target description
    phosphoglycerate kinase 1
  • Target synonyms
    cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; HEL-S-68p; MIG10; PGKA; phosphoglycerate kinase 1; primer recognition protein 2; PRP 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000282.1. Purification: Antigen affinity purified. Names and symbols: PGK1; phosphoglycerate kinase 1; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; primer recognition protein 2
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGK1 using anti-PGK1 antibody (PB9774). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK1 antigen affinity purified polyclonal antibody (Catalog # PB9774) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGK1 at approximately 43 kDa. The expected band size for PGK1 is at 43 kDa.
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