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Goat anti-PTGR1 Antibody

EB12936
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPTGR1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PTGR1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID22949
  • Target name
    PTGR1
  • Target description
    prostaglandin reductase 1
  • Target synonyms
    DIG-1, LTB4DH, PGR1, ZADH3, prostaglandin reductase 1, 15-oxoprostaglandin 13-reductase, D3T-inducible gene 1 protein, NAD(P)H-dependent alkenal/one oxidoreductase, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PRG-1, dithiolethione-inducible gene 1 protein, leukotriene B4 12-hydroxydehydrogenase, zinc binding alcohol dehydrogenase domain containing 3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036344.2; NP_001139581.1. Purification: Antigen affinity purified. Names and symbols: PTGR1; prostaglandin reductase 1; LTB4DH; PGR1; ZADH3; 15-oxoprostaglandin 13-reductase; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; PRG-1; leukotriene B4 12-hydroxydehydrogenase; zinc binding alcohol dehydrogenase domain containing 3
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (A08587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human Hacat whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (Catalog # A08587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.
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