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Western Blot analysis of HT29 cells using LTB4DH Polyclonal Antibody
Western Blot analysis of HT29 cells using LTB4DH Polyclonal Antibody
Western Blot analysis of HT29 cells using LTB4DH Polyclonal Antibody

PTGR1 Antibody

CSB-PA003169
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPTGR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22949
  • Target name
    PTGR1
  • Target description
    prostaglandin reductase 1
  • Target synonyms
    DIG-1, LTB4DH, PGR1, ZADH3, prostaglandin reductase 1, 15-oxoprostaglandin 13-reductase, D3T-inducible gene 1 protein, NAD(P)H-dependent alkenal/one oxidoreductase, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PRG-1, dithiolethione-inducible gene 1 protein, leukotriene B4 12-hydroxydehydrogenase, zinc binding alcohol dehydrogenase domain containing 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14914
  • Protein Name
    Prostaglandin reductase 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (A08587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human Hacat whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (Catalog # A08587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.
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