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ProductsBack/PTGR1 antibody [N1C2]

PTGR1 antibody [N1C2]

GTX118527
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPTGR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PTGR1 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.93 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22949
  • Target name
    PTGR1
  • Target description
    prostaglandin reductase 1
  • Target synonyms
    DIG-1, LTB4DH, PGR1, ZADH3, prostaglandin reductase 1, 15-oxoprostaglandin 13-reductase, D3T-inducible gene 1 protein, NAD(P)H-dependent alkenal/one oxidoreductase, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PRG-1, dithiolethione-inducible gene 1 protein, leukotriene B4 12-hydroxydehydrogenase, zinc binding alcohol dehydrogenase domain containing 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14914
  • Protein Name
    Prostaglandin reductase 1
  • Scientific Description
    This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Herrera-López EE, Guerrero-Escalera D, Aguirre-Maldonado I, et al. Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis. Sci Rep. 2023,13(1):6948. doi: 10.1038/s41598-023-34117-8
    Read this paper

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Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (A08587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human Hacat whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (Catalog # A08587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.
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