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Goat anti-RANBP1

EB06420
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRANBP1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RANBP1
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5902
  • Target name
    RANBP1
  • Target description
    RAN binding protein 1
  • Target synonyms
    HTF9A, ran-specific GTPase-activating protein, HpaII tiny fragments locus 9A, testis secretory sperm-binding protein Li 221n
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002873.1; NP_001265568.1; NP_001265569.1; NP_001265570.1. Purification: Antigen affinity purified. Names and symbols: RANBP1; RAN binding protein 1; HTF9A; MGC88701
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of RanBP1 using anti-RanBP1 antibody (A04000-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RanBP1 antigen affinity purified polyclonal antibody (Catalog # A04000-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RanBP1 at approximately 27KD. The expected band size for RanBP1 is at 23KD.
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