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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA019308HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA019308HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA019308HA01HU at dilution of 1:100

RANBP1 Antibody

CSB-PA019308HA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRANBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RANBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5902
  • Target name
    RANBP1
  • Target description
    RAN binding protein 1
  • Target synonyms
    HpaII tiny fragments locus 9A; HTF9A; ran-specific GTPase-activating protein; testis secretory sperm-binding protein Li 221n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43487
  • Protein Name
    Ran-specific GTPase-activating protein
  • Scientific Description
    Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RanBP1 Polyclonal Antibody, Unconjugated (bs-9322R) at 1:400 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of RanBP1 using anti-RanBP1 antibody (A04000-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RanBP1 antigen affinity purified polyclonal antibody (Catalog # A04000-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RanBP1 at approximately 27KD. The expected band size for RanBP1 is at 23KD.
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