![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103192/GTX103192_39932_20230331_WB_23041023_283.webp)
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RanBP1 antibody [N1C3]
GTX103192
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRANBP1
Overview
- SupplierGeneTex
- Product NameRanBP1 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5902
- Target nameRANBP1
- Target descriptionRAN binding protein 1
- Target synonymsHTF9A, ran-specific GTPase-activating protein, HpaII tiny fragments locus 9A, testis secretory sperm-binding protein Li 221n
- HostRabbit
- IsotypeIgG
- Protein IDP43487
- Protein NameRan-specific GTPase-activating protein
- Scientific DescriptionRan/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chao HW, Lai YT, Lu YL, et al. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3. Nucleic Acids Res. 2012,40(17):8484-98.Read this paper


![Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103192/GTX103192_39932_20180202_WB_KO_watermark_w_23060119_504.webp)
![Ran BP1 antibody [N1C3] detects Ran BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ran BP1 protein stained by Ran BP1 antibody [N1C3] (GTX103192) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining. Ran BP1 antibody [N1C3] detects Ran BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ran BP1 protein stained by Ran BP1 antibody [N1C3] (GTX103192) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103192/GTX103192_39932_20150410_IFA_w_23060119_466.webp)

![RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RanBP1 stained by RanBP1 antibody [HL2358] (GTX638564) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638564/GTX638564_T-45033_20230602_ICC_IF_23060622_893.webp)






