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Goat anti-RNF8

EB05843
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetRNF8
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RNF8 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9025
  • Target name
    RNF8
  • Target description
    ring finger protein 8
  • Target synonyms
    C3HC4-type zinc finger protein; E3 ubiquitin-protein ligase RNF8; hRNF8; ring finger protein (C3HC4 type) 8; ring finger protein 8, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF8; UBC13/UEV-interacting ring finger protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003949.1; NP_898901.1. Purification: Antigen affinity purified. Names and symbols: RNF8; ring finger protein 8; ring finger protein (C3HC4 type) 8; KIAA0646; FLJ12013; C3HC4-type zinc finger protein; OTTHUMP00000039684; UBC13/UEV-interacting ring finger protein;
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF8 using anti-RNF8 antibody (A00707-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF8 antigen affinity purified polyclonal antibody (Catalog # A00707-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF8 at approximately 56 kDa. The expected band size for RNF8 is at 56 kDa.
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