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Goat anti-SAE2 / UBA2

EB06098
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetUBA2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SAE2 / UBA2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10054
  • Target name
    UBA2
  • Target description
    ubiquitin like modifier activating enzyme 2
  • Target synonyms
    anthracycline-associated resistance ARX; ARX; HRIHFB2115; SAE2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1 homolog; ubiquitin-like 1-activating enzyme E1B
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005490. GeneIDs all Nonhuman: 50995 (mouse);. Purification: Antigen affinity purified. Names and symbols: UBA2; SAE2; SUMO-1 activating enzyme subunit 2
  • Reactivity Supplier
    Human, Mouse, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system. Kanemaru A et al., 2013, Biosci Biotechnol Biochem
    Read more

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Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human 22RV1 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody (Catalog # A03816-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.
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