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Western Blot analysis of 293 cells using UBA2 Polyclonal Antibody
Western Blot analysis of 293 cells using UBA2 Polyclonal Antibody
Western Blot analysis of 293 cells using UBA2 Polyclonal Antibody

UBA2 Antibody

CSB-PA004355
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUBA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    UBA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10054
  • Target name
    UBA2
  • Target description
    ubiquitin like modifier activating enzyme 2
  • Target synonyms
    anthracycline-associated resistance ARX; ARX; HRIHFB2115; SAE2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1 homolog; ubiquitin-like 1-activating enzyme E1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBT2
  • Protein Name
    SUMO-activating enzyme subunit 2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human 22RV1 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody (Catalog # A03816-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.
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