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ProductsBack/Goat anti-Synaptotagmin I

Goat anti-Synaptotagmin I

EB08253
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSYT1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Synaptotagmin I Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6857
  • Target name
    SYT1
  • Target description
    synaptotagmin 1
  • Target synonyms
    BAGOS, P65, SVP65, SYT, synaptotagmin-1, synaptotagmin I, sytI
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005630.1. GeneIDs all Nonhuman: 20979 (mouse); 25716 (rat);. Purification: Antigen affinity purified. Names and symbols: synaptotagmin I ; DKFZp781D2042; SYT1; P65; SVP65; SYT; synaptotagmin 1
  • Reactivity Supplier
    Human, Mouse, Rat. Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody (Catalog # A02314-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.
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