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Western Blot analysis of 293 cells using Synaptotagmin Polyclonal Antibody
Western Blot analysis of 293 cells using Synaptotagmin Polyclonal Antibody
Western Blot analysis of 293 cells using Synaptotagmin Polyclonal Antibody

SYT1/SYT2 Antibody

CSB-PA004203
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSYT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SYT1/SYT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6857
  • Target name
    SYT1
  • Target description
    synaptotagmin 1
  • Target synonyms
    BAGOS, P65, SVP65, SYT, synaptotagmin-1, synaptotagmin I, sytI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21579
  • Protein Name
    Synaptotagmin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody (Catalog # A02314-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.
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