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ProductsBack/Synaptotagmin 1 antibody [N1C2]

Synaptotagmin 1 antibody [N1C2]

GTX114124
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSYT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Synaptotagmin 1 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6857
  • Target name
    SYT1
  • Target description
    synaptotagmin 1
  • Target synonyms
    BAGOS; P65; SVP65; synaptotagmin I; synaptotagmin-1; SYT; sytI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21579
  • Protein Name
    Synaptotagmin-1
  • Scientific Description
    The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody (Catalog # A02314-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.
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