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Goat anti-SYVN1

EB09821
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetSYVN1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SYVN1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and endoplasmic reticulum of A431 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID84447
  • Target name
    SYVN1
  • Target description
    synoviolin 1
  • Target synonyms
    DER3; E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1; RING-type E3 ubiquitin transferase synoviolin; synovial apoptosis inhibitor 1, synoviolin
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_115807.1; NP_757385.1. GeneIDs all Nonhuman: 361712 (rat);. Purification: Antigen affinity purified. Names and symbols: SYVN1; synovial apoptosis inhibitor 1, synoviolin; HRD1; KIAA1810; MGC40372; synoviolin 1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HRD1/SYVN1 using anti-HRD1/SYVN1 antibody (A02670-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRD1/SYVN1 antigen affinity purified polyclonal antibody (Catalog # A02670-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRD1/SYVN1 at approximately 76 kDa. The expected band size for HRD1/SYVN1 is at 68 kDa.
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