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ProductsBack/SYVN1 Polyclonal Antibody
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti SYVN1/HRD1 Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti SYVN1/HRD1 Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti SYVN1/HRD1 Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

SYVN1 Polyclonal Antibody

BS-0679R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSYVN1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SYVN1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID84447
  • Target name
    SYVN1
  • Target description
    synoviolin 1
  • Target synonyms
    DER3; E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1; RING-type E3 ubiquitin transferase synoviolin; synovial apoptosis inhibitor 1, synoviolin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86TM6
  • Protein Name
    E3 ubiquitin-protein ligase synoviolin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway. Dong JY et al., 2021 Sep, Acta Pharmacol Sin
    Read more
  • Experimental study of the protective effects of SYVN1 against diabetic retinopathy. Yang S et al., 2015 Sep 11, Sci Rep
    Read more

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Figure 1. Western blot analysis of HRD1/SYVN1 using anti-HRD1/SYVN1 antibody (A02670-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRD1/SYVN1 antigen affinity purified polyclonal antibody (Catalog # A02670-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRD1/SYVN1 at approximately 76 kDa. The expected band size for HRD1/SYVN1 is at 68 kDa.
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