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TIM3 Antibody (YA1448, Mouse)

HY-P81703
MedChem Express
TargetHAVCR2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TIM3 Antibody (YA1448, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID84868
  • Target name
    HAVCR2
  • Target description
    hepatitis A virus cellular receptor 2
  • Target synonyms
    CD366; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; Tim-3; TIMD3; TIMD-3
  • Host
    Mouse
  • Isotype
    IgG
  • Scientific Description
    TIM3 Antibody (YA1448) is a mouse-derived non-conjugated IgG antibody (Clone NO.: YA1448), targeting TIM3. TIM3 Antibody (YA1448) can be used for WB, IHC-P, IF-Cell, FC experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
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