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Goat Anti-Type IV Collagen-BIOT

1340-08
SouthernBiotech
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL4A1
Price on request
0.2 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Goat Anti-Type IV Collagen-BIOT
  • Delivery Days Customer
    7
  • Applications
    Electron Microscopy, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID1282
  • Target name
    COL4A1
  • Target description
    collagen type IV alpha 1 chain
  • Target synonyms
    arresten; BSVD; BSVD1; COL4A1 NC1 domain; COL4A1s; collagen alpha-1(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; PADMAL; RATOR
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP02462
  • Protein Name
    Collagen alpha-1(IV) chain
  • Scientific Description
    Pooled antisera from goats hyperimmunized with type IV collagen; purified by affinity chromatography on type IV collagen covalently linked to agarose; reacts with conformational determinants on type IV collagen; cross-adsorbed against Collagen types I, II, III, V, and VI for minimal reactivity
  • Source
    Pooled antisera from goats hyperimmunized with type IV collagen
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.
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