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ProductsBack/Goat anti-XBP1 / TREB5

Goat anti-XBP1 / TREB5

EB08557
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetXBP1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-XBP1 / TREB5 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7494
  • Target name
    XBP1
  • Target description
    X-box binding protein 1
  • Target synonyms
    tax-responsive element-binding protein 5; TREB5; TREB-5; X-box-binding protein 1; XBP-1; XBP2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001073007.1. GeneIDs all Nonhuman: 22433 (mouse);. Purification: Antigen affinity purified. Names and symbols: XBP1; TREB5; X-box binding protein 1 ; XBP2; OTTHUMP00000028682; X-box-binding protein-1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Testis lysates; Lane 4: HepG2 cell lysates probed with XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of XBP using anti-XBP antibody (PB9463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: MM231 Whole Cell Lysate at 40ug, Lane 3: MM453 Whole Cell Lysate at 40ug, Lane 4: SKOV Whole Cell Lysate at 40ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP antigen affinity purified polyclonal antibody (Catalog # PB9463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP at approximately 29 kDa. The expected band size for XBP is at 29 kDa.
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