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WB analysis of HeLa lysate with (B) and without (A) blocking with the immunising peptide using GTX89474 GOLGA3 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HeLa lysate with (B) and without (A) blocking with the immunising peptide using GTX89474 GOLGA3 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HeLa lysate with (B) and without (A) blocking with the immunising peptide using GTX89474 GOLGA3 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

GOLGA3 antibody, C-term

GTX89474
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetGOLGA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    GOLGA3 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2802
  • Target name
    GOLGA3
  • Target description
    golgin A3
  • Target synonyms
    GCP170; golgi autoantigen, golgin subfamily a, 3; golgi complex-associated protein of 170 kDa; Golgi membrane associated protein; Golgi peripheral membrane protein; golgin subfamily A member 3; golgin-160; golgin-165; male enhanced antigen-2; MEA-2; SY2/SY10 protein
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ08378
  • Protein Name
    Golgin subfamily A member 3
  • Scientific Description
    The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Feb 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA3 antigen affinity purified polyclonal antibody (Catalog # A07522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLGA3 at approximately 180 kDa. The expected band size for GOLGA3 is at 167 kDa.
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