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Western blot analysis of extracts of various cell lines using GOLPH3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using GOLPH3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using GOLPH3 Polyclonal Antibody at dilution of 1:3000.

GOLPH3 Polyclonal Antibody

E-AB-64467
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    GOLPH3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH3 antigen affinity purified polyclonal antibody (Catalog # A01333-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH3 at approximately 36 kDa. The expected band size for GOLPH3 is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-GOLPH3 Antibody Picoband(r)A01333-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGOLPH3
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Product group Antibodies
RayBiotech
Anti-GOLPH3 Antibody144-60200
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGOLPH3
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Product group Antibodies
Biomatik
Golph3 Polyclonal AntibodyCAC11220
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetGOLPH3
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Hela cell lysates probed with GOLPH3 Polyclonal Antibody, Unconjugated (bs-13488R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
GOLPH3 Polyclonal AntibodyBS-13488R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Rat
TargetGOLPH3
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Product group Antibodies
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GOLPH3 AntibodyCAB13121
ReactivityHuman
TargetGOLPH3
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Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-GOLPH3 AntibodyHPA044564
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetGOLPH3
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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: GOLPH3 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Product group Antibodies
Cusabio
GOLPH3 AntibodyCSB-PA872494LA01HU
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGOLPH3
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Product group Antibodies
Everest Biotech
Goat anti-GOLPH3 AntibodyEB09796
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse
TargetGOLPH3
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Sample (50 ug of whole cell lysate) A: mouse lung 10% SDS PAGE GTX122856 diluted at 1:3000
Product group Antibodies
GeneTex
GOLPH3 antibody [N1C3]GTX122856
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TargetGOLPH3
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Product group Antibodies
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GOLPH3 AntibodyLS-C748179
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TargetGOLPH3
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