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GOT1 Antibody Pair

CSB-EAP009679
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Equine, Human
TargetGOT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOT1 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.1ug/ml and the detection antibody at a concentration of 0.1ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID2805
  • Target name
    GOT1
  • Target description
    glutamic-oxaloacetic transaminase 1
  • Target synonyms
    AST, AST1, ASTQTL1, GIG18, SGOT, cAspAT, cCAT, aspartate aminotransferase, cytoplasmic, aspartate aminotransferase 1, aspartate transaminase 1, cysteine aminotransferase, cytoplasmic, cysteine transaminase, cytoplasmic, glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble, growth-inhibiting protein 18, testis secretory sperm-binding protein Li 196a, transaminase A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17174
  • Protein Name
    Aspartate aminotransferase, cytoplasmic
  • Reactivity
    Bovine, Equine, Human
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (A04085-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aspartate Aminotransferase/GOT1 antigen affinity purified polyclonal antibody (Catalog # A04085-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aspartate Aminotransferase/GOT1 at approximately 41KD. The expected band size for Aspartate Aminotransferase/GOT1 is at 46KD.
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