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ProductsBack/GOT2 Mouse Monoclonal Antibody [Clone ID: 3E9]
Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.

GOT2 Mouse Monoclonal Antibody [Clone ID: 3E9]

AM06383SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetGOT2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    GOT2 Mouse Monoclonal Antibody [Clone ID: 3E9]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3E9
  • Gene ID2806
  • Target name
    GOT2
  • Target description
    glutamic-oxaloacetic transaminase 2
  • Target synonyms
    DEE82, KAT4, KATIV, KYAT4, mitAAT, aspartate aminotransferase, mitochondrial, FABP-1, FABPpm, aspartate aminotransferase 2, aspartate transaminase 2, fatty acid-binding protein, glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial, kynurenine aminotransferase 4, kynurenine aminotransferase IV, kynurenine--oxoglutarate transaminase 4, kynurenine--oxoglutarate transaminase IV, mAspAT, plasma membrane-associated fatty acid-binding protein, transaminase A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP00505
  • Protein Name
    Aspartate aminotransferase, mitochondrial
  • Scientific Description
    GOT2 mouse monoclonal antibody, clone 3E9, Ascites
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FABP-1/GOT2 using anti-FABP-1/GOT2 antibody (A05998-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Caco-2 whole cell lysates, Lane 9: human U937 whole cell lysates, Lane 10: rat brain tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat kidney tissue lysates, Lane 13: rat ovary tissue lysates, Lane 14: mouse brain tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse kidney tissue lysates, Lane 17: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP-1/GOT2 antigen affinity purified polyclonal antibody (Catalog # A05998-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP-1/GOT2 at approximately 41KD. The expected band size for FABP-1/GOT2 is at 41KD.
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