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GP78 / AMFR Antibody

LS-C815879
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAMFR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GP78 / AMFR Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:50 - 1:200) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID267
  • Target name
    AMFR
  • Target description
    autocrine motility factor receptor
  • Target synonyms
    E3 ubiquitin-protein ligase AMFR; GP78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR; RNF45
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: AMFR antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
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Figure 1. Western blot analysis of AMFR using anti-AMFR antibody (PB10039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMFR antigen affinity purified polyclonal antibody (Catalog # PB10039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMFR at approximately 73 kDa. The expected band size for AMFR is at 73 kDa.
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