WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 ug of HEK293 whole cell lysate (lane 1). Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4?. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).