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ProductsBack/GSK3 beta antibody [3D10]
ICC/IF analysis of NIH3T3 (left) and U251 (right) cells using GTX83315 GSK3 beta antibody [3D10]. Green : GSK3 beta Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of NIH3T3 (left) and U251 (right) cells using GTX83315 GSK3 beta antibody [3D10]. Green : GSK3 beta Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of NIH3T3 (left) and U251 (right) cells using GTX83315 GSK3 beta antibody [3D10]. Green : GSK3 beta Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments

GSK3 beta antibody [3D10]

GTX83315
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetGSK3B
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Overview

  • Supplier
    GeneTex
  • Product Name
    GSK3 beta antibody [3D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D10
  • Conjugate
    Unconjugated
  • Gene ID2932
  • Target name
    GSK3B
  • Target description
    glycogen synthase kinase 3 beta
  • Target synonyms
    glycogen synthase kinase-3 beta, GSK-3 beta, serine/threonine-protein kinase GSK3B
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP49841
  • Protein Name
    Glycogen synthase kinase-3 beta
  • Scientific Description
    The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • GSK3beta negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons. Chien T et al., 2018 Dec, Mol Psychiatry
    Read this paper
  • GSK3beta expression and phosphorylation during neuronal maturation in the rat dorsal root ganglion. Tang W et al., 2015, Int J Clin Exp Med
    Read this paper

Datasheet

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Figure 1. Western blot analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta/GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 beta/GSK3B at approximately 46 kDa. The expected band size for GSK3 beta/GSK3B is at 46 kDa.
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