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IHC-P analysis of human breast carcinoma tissue using GTX86837 GSK3 beta (phospho Ser9) antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX86837 GSK3 beta (phospho Ser9) antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX86837 GSK3 beta (phospho Ser9) antibody. The picture on the right is blocked with the synthesized peptide.

GSK3 beta (phospho Ser9) antibody

GTX86837
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGSK3B
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Overview

  • Supplier
    GeneTex
  • Product Name
    GSK3 beta (phospho Ser9) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2932
  • Target name
    GSK3B
  • Target description
    glycogen synthase kinase 3 beta
  • Target synonyms
    glycogen synthase kinase-3 beta, GSK-3 beta, serine/threonine-protein kinase GSK3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49841
  • Protein Name
    Glycogen synthase kinase-3 beta
  • Scientific Description
    The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mietelska-Porowska A, Domańska J, Want A, et al. Induction of Brain Insulin Resistance and Alzheimer's Molecular Changes by Western Diet. Int J Mol Sci. 2022,23(9). doi: 10.3390/ijms23094744
    Read this paper
  • Kuo YH, Chen CC, Wu PY, et al. N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms. BMC Complement Altern Med. 2017,17(1):71. doi: 10.1186/s12906-016-1554-6
    Read this paper
  • Lin HC, Lin MH, Liao JH, et al. Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets. J Agric Food Chem. 2017,65(1):51-59. doi: 10.1021/acs.jafc.6b04101
    Read this paper
  • Chen MJ, Wu DW, Wang YC, et al. PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness. Sci Rep. 2016,6:34933. doi: 10.1038/srep34933
    Read this paper
  • Wu JY, Tsai KW, Li YZ, et al. Anti-Bladder-Tumor Effect of Baicalein from Scutellaria baicalensis Georgi and Its Application In Vivo. Evid Based Complement Alternat Med. 2013,2013:579751. doi: 10.1155/2013/579751
    Read this paper
  • Lin YS, Chuang MT, Chen CH, et al. Nicotinic acid hydroxamate downregulated the melanin synthesis and tyrosinase activity through activating the MEK/ERK and AKT/GSK3β signaling pathways. J Agric Food Chem. 2012,60(19):4859-64. doi: 10.1021/jf301109p
    Read this paper

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Figure 1. Western blot analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta/GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 beta/GSK3B at approximately 46 kDa. The expected band size for GSK3 beta/GSK3B is at 46 kDa.
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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-GSK-3 Beta Polyclonal Antibody (bs-0028R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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