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GSR Polyclonal Antibody

RD77440A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGSR
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    GSR Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2936
  • Target name
    GSR
  • Target description
    glutathione-disulfide reductase
  • Target synonyms
    CNSHA10, GR, GSRD, HEL-75, HEL-S-122m, glutathione reductase, mitochondrial, GRase, epididymis luminal protein 75, epididymis secretory sperm binding protein Li 122m, glutathione S-reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00390
  • Protein Name
    Glutathione reductase, mitochondrial
  • Scientific Description
    This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. - This is a GSR Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse colon tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Reductase antigen affinity purified polyclonal antibody (Catalog # A01479-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Reductase at approximately 52 kDa. The expected band size for Glutathione Reductase is at 56 kDa.
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